Guests will be able to stay overnight in one of nine cabins and enjoy exclusive early morning and evening tours of the facility
Each lodge can accommodate two adults. On family nights, a maximum of four people are welcome, including up to three children aged between five and 13.
Tickets go on sale on Saturday and the first overnight stays begin in May next year.
Land of the Lions will reopen next spring, and will provide state-of-the-art facilities for a breeding group of endangered Asiatic lions, of which only 400 remain in the wild.
It has been inspired by the landscape of the Gir Forest and will be five times the size of the previous enclosure
Asiatic lions will sleep for up to 16 hours-a-day, and visitors will be able to see them enjoying their cat-naps in their custom-built "lion hotel".
Photo: ZSL London Zoo
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The enclosure will feature a bustling Indian "village" to enable guests to experience the vibrant culture of the lion’s native home, before they reach the lions' den, featuring an Indian train station, a conservation centre for rangers and an abandoned ancient fort.
The enclosure will enable the big cats to claim areas as their own, which they do by scent marking and vocalisations – a mighty roar which can be heard from miles away.
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Emma Taylor, head of product development, told the Daily Mail: "London Zoo is a magical place at night - we wanted people of all ages to have a chance to enjoy that magic, and immerse themselves in a fantastic experience unlike any other, sleeping near to our magnificent Asiatic lions.
Photo: ZSL London Zoo
"It's hard to believe you're in the heart of the capital when you're surrounded by the noises of the wild.
"Staying overnight at the Gir Lion Lodge will give guests the chance to explore the zoo on a hosted tour, while helping to fund our work for wildlife around the world."
Reports of drunken visitors at the zoo's "lates" events last year raused questions about whether the zoo was focusing too much on profits rather than conservation.
Vistors were said to have disturbed the animals by throwing beer at tigers or trying to get in pens with animals.